HomeFAQsWhat is the difference between Exonuclease VII and Exonuclease I (NEB# M0293)?
FAQ: What is the difference between Exonuclease VII and Exonuclease I (NEB# M0293)?
Exonuclease VII can degrade ssDNA from both directions unlike Exonuclease I which works only from the 3' to 5' direction. Exonuclease VII can also degrade both 5' or 3' terminally phosphorothioated ssDNA-oligos. Exonuclease I is blocked by the presence of 3’ phosphorothioates.
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