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  • FAQ: Does the PCR product need to be purified?

    No, although higher transformation efficiencies are achieved with purified in serts. If using unpurified PCR amplicons, use 1 μl or less of the PCR as insert material to achieve a 3:1 molar ratio of insert:vector. It is especially important for high yield PCR situations to avoid adding a vast excess of insert that would result in the insert ligating to both ends of the vector backbone.