HomeFAQsDo my inserts have to possess 5´ phosphates?
FAQ: Do my inserts have to possess 5´ phosphates?
No, this cloning strategy works well with inserts whether or not they possess 5´ phosphates. In fact, using non-phosphorylated primers for PCR eliminates the possibility of multiple inserts being cloned into the pMiniT 2.0 vector.
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