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  • FAQ: Does Bst 2.0 DNA polymerase have reverse transcriptase activity?

    Yes. To use for RT applications we recommend elevated magnesium concentrations (~8 mM). Bst 2.0 exhibits higher amplification variability with RNA templates, as some primer sets will perform more efficiently than others. For more robust and consistent RT reactions we recommend a dedicated reverse transcriptase, but Bst 2.0 alone is recommended for single enzyme applications although optimization may be required.