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  • FAQ: What is the effect of reducing agents on rSAP activity?

    Reducing agents, DTT or β-mercaptoethanol (β-ME), may decrease rSAP activity:

    Reducing
    Agents
    Concentration Activity
    DTT 0 mM 100%
    1 mM 88%
    5 mM 63%
    10 mM 46%
    50 mM 20%
    β-ME 0 mM 100%
    5 mM 95%
    10 mM 78%
    20 mM 75%

    rSAP activity was measured by PNPP assay in 1X rSAP Reaction Buffer supplemented with varying amounts of DTT or β-ME. Activity is shown as percent activity compared to the control, which did not contain added reducing agents.