# FAQ: What is the effect of reducing agents on rSAP activity?

Reducing agents, DTT or β-mercaptoethanol (β-ME), may decrease rSAP activity:

 Reducing Agents Concentration Activity DTT 0 mM 100% 1 mM 88% 5 mM 63% 10 mM 46% 50 mM 20% β-ME 0 mM 100% 5 mM 95% 10 mM 78% 20 mM 75%

rSAP activity was measured by PNPP assay in 1X rSAP Reaction Buffer supplemented with varying amounts of DTT or β-ME. Activity is shown as percent activity compared to the control, which did not contain added reducing agents.