FAQ: What is the effect of reducing agents on rSAP activity?

Reducing agents, DTT or β-mercaptoethanol (β-ME), may decrease rSAP activity:

Concentration Activity
DTT 0 mM 100%
1 mM 88%
5 mM 63%
10 mM 46%
50 mM 20%
β-ME 0 mM 100%
5 mM 95%
10 mM 78%
20 mM 75%

rSAP activity was measured by PNPP assay in 1X rSAP Reaction Buffer supplemented with varying amounts of DTT or β-ME. Activity is shown as percent activity compared to the control, which did not contain added reducing agents.