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  • FAQ: What is the difference between the NEBNext Ultra Ligation Module and the NEBNext Quick Ligation Module?

    The NEBNext Ultra Ligation Module contains the ligation components that are designed to be used with the new Ultra kit workflows (Blunt/TA Ligase Master Mix plus NEBNext Ligation Enhancer). The Ultra Ligation Module components have been validated only with the Ultra workflow. The Quick Ligation module contains Quick T4 DNA Ligase and Quick Ligation Reaction Buffer (5X) and has not been validated in the Ultra workflow.