HomeFAQsCan I display antibody fragments or proteins at the N-terminus of pIII coat protein with M13KE?
FAQ: Can I display antibody fragments or proteins at the N-terminus of pIII coat protein with M13KE?
In the Ph.D.™ phage display system each copy of pIII displays the encoded peptide sequence. For this reason any insert that interferes with phage particle assembly or pIII function will not make viable phage. Typically phagemid systems, where a second copy of native coat protein is provided on by helper phage, are used to display protein-sized inserts on M13. We consider 25-50 amino acids to be the maximum range for this system. To some extent it is also sequence and secondary structure dependent.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.