FAQ: What is the binding capacity of the Magnetic Chitin Beads used to remove Endo S?

The Magnetic Chitin Beads (NEB# E8036) binding capacity is approximately 0.4mg/ml of CBD-tagged protein. This binding capacity is calculated in mg of protein per bed volume of resin. The chitin magnetic beads are a 50:50 slurry. Therefore, 50 μl of slurry will yield 25 μl bed volume of resin, which is enought to remove 1-5 µL of Endo S.