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  • FAQ: What is a typical Fetuin deglycosylation reaction condition?

    1. Combine 2 μl of Fetuin, 1 μl of 10X Glycoprotein Denaturing Buffer, and 5 μl of H2O.
    2. Incubate at 95°C for 10 minutes.
    3. Add 1 μl of 10X G7 Reaction Buffer and 1 μl of 10% NP-40.
    4. Add 1 μl of endoglycosidase.
    5. Incubate at 37°C for 1 hour.
    6. Visualize reaction products on 10–20% SDS-PAGE gel.

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