We recommend loading 1ug (2ul) of the Lambda DNA-Hind III. The fragments can be separated effectively on agarose gels ranging from 0.6 to 1% using TBE or TAE buffers. To prepare the samples, put 2ul of the marker into 13ul TE buffer (10mM Tris-HCl, 1mM EDTA) and add 3ul of 6X loading buffer for a final volume of 18ul. Heat the samples to 60C for 3 minutes and remove to ice or load immediately on the gel. Loading more than 1ug will result in the larger bands appearing very broad and therefore more difficult to use for accurate sizing. Loading less than 0.5ug makes the smaller bands harder to visualize.