FAQ: How can the yield of RNA be maximized when using SP6 RNA Polymerase?

Higher yields of RNA may be obtained by raising NTP concentrations (up to 4 mM each). Mg2+ concentration should be raised to 4 mM above the total NTP concentration. Additionally, inorganic pyrophosphatase (NEB# M2403 ) can be added to a final concentration of 4 units/ml to lower feedback inhibition.