NEB will be closed Thursday, November 23, 2017 and Friday, November 24, 2017. Orders placed will process Monday, November 27, 2017 for delivery on Tuesday, November 28,2017.

FAQ: If I accidentally move all 30 ul of the 5’ ligation product forward to the reverse transcription mixture will the reaction still work?

Moving all of the 5’ ligation reaction product into the reverse transcription reaction will greatly reduce the activity of the M-MuLV (RNase H-) Reverse Transcriptase due to the elevated levels of Mg. Amplification output will be only ~15% of what would have been attained with 14 ul of 5’ ligation product.
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