FAQ: For some restriction enzymes star activity can be a concern. Is this also applicable to the RE-Mix?

Star activity in general is not a concern with RE-Mix as the recommended incubation time is short, the amount of glycerol is not more than 5% and the restriction enzymes used in the RE-Mix are not known to be prone to star activity. However, as is true with any restriction enzyme, extended digests, high glycerol and high enzyme concentration can lead to star activity.