HomeFAQsCan Bst 2.0 DNA Polymerase be used to fill in 3' overhangs?
FAQ: Can Bst 2.0 DNA Polymerase be used to fill in 3' overhangs?
No, DNA polymerases cannot fill in 3' overhangs. To create blunt ends 3' overhangs must be removed. DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210), T4 DNA Polymerase (NEB# M0203) and Mung Bean Nuclease (NEB# M0250) are the best choices to remove 3' overhangs.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.