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HomeFAQsThe recommended volume for an Instant Sticky-end Ligase Master Mix reaction is 10ul. I like to set-up my ligation reactions in a 20 ul volume. Can I scale-up the reaction?
FAQ: The recommended volume for an Instant Sticky-end Ligase Master Mix reaction is 10ul. I like to set-up my ligation reactions in a 20 ul volume. Can I scale-up the reaction?
Yes. As long as the concentration of the Instant Sticky-end Ligase Master Mix in the reaction is 1X (or 50% of the total reaction volume), you can scale up the ligation reaction.
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