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  • FAQ: What is the composition of the ThermoPol™ DF (detergent-free) buffer?

    1X ThermoPol DF (detergent-free) Reaction Buffer: 20 mM Tris-HCl, 10 mM (NH4)2SO4, 10 mM KCl, 2 mM MgSO4, pH 8.8 @ 25°C. Supplied as a 10X concentrated stock. For reactions requiring dNTPs, Deoxynucleotide Solution Mix (NEB #N0447), or Deoxynucleotide Solution Set (NEB #N0446) are available separately.