FAQ: Is the Mung Bean Nuclease active in other NEBuffers?

Mung Bean Nuclease is also fully active CutSmart® Buffer. The enzyme may also be used in NEBuffers 1, 2, 4 ,1.1, 2.1 however the reaction time should be doubled to 1 hour at 30°C when these buffers are used. Activity is poor in NEBuffers 3 and 3.1.