Fourth of July Shipping Notice: Orders placed on Friday, July 1, 2022, will be shipped on Tuesday, July 5, 2022, for delivery on Wednesday, July 6, 2022.
HomeFAQsIs there a difference in cutting close to the ends between NotI-HF and NotI?
FAQ: Is there a difference in cutting close to the ends between NotI-HF and NotI?
No. When tested on a series of five oligos (19-23 bases in length) containing the restriction site and an additional 1 to 5 A/T bases from the end, both enzymes cut the oligo with 1 base pair from the end. When designing primers, NEB recommends adding 6 extra bases to ensure against experimental failure due to variation in substrate and reaction conditions.