FAQ: How does Hinp1I compare to HhaI?

HinP1I cleaves to leave a different overhang. HinP1I preoduces a 5´ extension, whereas HhaI produces a 3´ extension. The 5´ extension can be efficiently ligated into the AccI site of M13 and pUC cloning vectors. Both are blocked by CpG methylation.

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