HomeFAQsCan I end-label a polyadenylated RNA molecule with a Cy3/5 or biotin-modified base using the E. coli Poly(A) Polymerase?
FAQ: Can I end-label a polyadenylated RNA molecule with a Cy3/5 or biotin-modified base using the E. coli Poly(A) Polymerase?
Yes. Biotin-ATP can be incorporated efficiently by the Poly (A) Polymerase. Cy3/5 modified bases can also be incorporated, but at a lower efficiency. The tailing length and biotin density can be controlled by optimizing the reaction time, the ATP/biotin-ATP ratio and the total ATP concentration.
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