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  • FAQ: Are there any published papers in which McrBC has been used?

    Yes:
    Holemon, H. et al. (2007) “MethylScreen: DNA methylation density monitoring using quantitative PCR”. Biotechniques, 43, 683-93.

    Ordway, J. M. et al. (2007) “Identification of novel high-frequency DNA methylation changes in breast cancer”. PLoS ONE, 2, e1314.

    Vaughn, M. W. et al. (2007) “Epigenetic Natural Variation in Arabidopsis thaliana”. PLoS Biol. 5, e174.

    Ordway, J. M. et al. (2006) “Comprehensive DNA methylation profiling in a human cancer genome identifies novel epigenetic targets”. Carcinogenesis, 27, 2409-23.

    Rangwala, S. H. et al. (2006) “Meiotically stable natural epialleles of Sadhu, a novel Arabidopsis retroposon”. PLoS Genet. 2, e36.

    Schumacher, A. et al. (2006) “Microarray-based DNA methylation profiling: technology and applications”. Nucleic Acids Res. 34, 528-42.

    Smith, M. J., and Jeddeloh, J. A. (2005) “DNA methylation in lysogens of pathogenic Burkholderia spp. requires prophage induction and is restricted to excised phage DNA”. J. Bacteriol. 187, 1196-200.

    Yamada, Y. H. et al. (2004) “A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 21q”. Genome Res. 14, 247-66.

    Rabinowicz, P. D. et al. (2003) “Genes and transposons are differentially methylated in plants, but not in mammals”. Genome Res. 13, 2658-64.

    Pietrobono et al. (2002). "Quantitative analysis of DNA demethylation and transcriptional reactivation of the FMR1 gene in fragile X cells treated with 5-azadeoxycytidine". NAR, 30, 3278-3285.

    Rosado Fantappie et al. (2001). "Lack of DNA Methylation in Schistosoma mansoni". Exp Parasitol. 98, 162-166. [Note: Incorrect source of McrBC stated in this paper. McrBC is available ONLY from NEB.]

    Gowher et al. (2000). "DNA of Drosophila melanogaster contains 5-methylcytosine". EMBO 19, 6918 - 6923.

    Lyko et al. (2000). "Mammalian (cytosine-5) methyltransferases cause genomic DNA methylation and lethality in Drosophila". Nature Genetics 23, 363 - 366.

    Pradhan, S. and Roberts, R. J. (2000) “Hybrid mouse-prokaryotic DNA (cytosine-5) methyltransferases retain the specificity of the parental C-terminal domain”. EMBO J. 19, 2103-14.

    Santoso et al. (2000). "Control of organ-specific demethylation by an element of the T-cell receptor-alpha locus control region". J.Biol. Chem. 275, 1952-1958.

    Burman et al. (1999). "Hypomethylation of an expanded FMR1 allele is not associated with a global DNA methylation deficit". Am. J. Hum. Genet. 65, 1375-1386.

    Chotai and Payne (1998). "A rapid, PCR-based test for differential molecular diagnosis of Prader-Willi and Angelman syndromes:. J. Med. Genet. 35, 472-475.