HomeFAQsDoes the DNA need to be purified after the restriction digest, prior to Antarctic Phosphatase treatment?
FAQ: Does the DNA need to be purified after the restriction digest, prior to Antarctic Phosphatase treatment?
In most cases Antarctic Phosphatase will work well in a reaction containing the restriction enzyme but only when supplemented with 10X Antarctic Phosphatase Reaction Buffer to a final concentration of 1X. Heat inactivate the restriction enzyme if possible.
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