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HomeFAQsCan I use T4 Polynucleotide Kinase and T4 DNA Ligase in the same reaction buffer?
FAQ: Can I use T4 Polynucleotide Kinase and T4 DNA Ligase in the same reaction buffer?
Yes, for cold phosphorylations, but not for hot labeling reactions since the cold ATP in T4 Ligase buffer will interfere with labeling.
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