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  • FAQ: What are the mutations in your lambda DNA?

    Our Lambda DNA contains a total of 4 mutations:
        -ind1 at 37,589  C->T, which creates a Hind III site at 37,584.
        -Sam7 at 45,352 G->A.
    Sam7 is an amber mutation in a gene involved in lysis of the bacterial cell membrane. The absence of a wild-type S gene product leads to an intracellular accumulation of infectious bacteriophage particles.

        -cI857 at 37,742 C->T.
    cI857 is a temperature sensitive mutation that renders the CI gene product thermo labile.
        -cI857s7 strain also carries a G->A mutation at 43,082.