HomeFAQsWhy are there larger than expected bands after digestion with the KpnI-HF restriction enzyme?
FAQ: Why are there larger than expected bands after digestion with the KpnI-HF restriction enzyme?
The increased specificity for the KpnI-HF cut site has increased binding of the enzyme to the DNA and the enzyme may remain attached to the DNA during gel electrophoresis. To disrupt binding, add SDS to a final concentration of 0.5% - 1% or purify DNA before electrophoresis.
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