FAQ: Can E. coli Poly(A) Polymerase be used to add a poly A tail to ssDNA?

No. E. coli poly(A) polymerase will not add A's to single-stranded DNA. However, Terminal Transferase (M0315) is a DNA polymerase that will add a poly dA tail to the 3' end of single stranded DNA using dATP. Please note that Terminal Transferase will not use ATP.