HomeFAQsThe repaired DNA will be used for an Nsp1 or Sty1 digestion followed by an adapter ligation, and PCR. Do you recommend cleanup of the PreCR Repair Mix reaction prior to this process?
FAQ: The repaired DNA will be used for an Nsp1 or Sty1 digestion followed by an adapter ligation, and PCR. Do you recommend cleanup of the PreCR Repair Mix reaction prior to this process?
If the polymerase in the PreCR Repair Mix is still present and active when the DNA ends are generated by restriction enzyme digest, it may modify those ends (either by filling in the overhang or adding a non-templated dA in a manner similar to Taq DNA Polymerase) and interfere with the subsequent steps. A heat kill step (80oC for 20 minutes) or a DNA clean-up step is therefore recommended. The DNA can then be either ethanol precipitated or purified by the use of a spin-column.
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