HomeFAQsDoes the DNA need to be purified after the restriction digest, prior to CIP treatment?
FAQ: Does the DNA need to be purified after the restriction digest, prior to CIP treatment?
In most cases, CIP will work well in the reaction containing the restriction enzyme. Heat inactivate the restriction enzyme if possible. Some enzymes may bind tightly to the DNA ends and compete with CIP. Check the restriction enzyme data card for information regarding excessive DNA binding.
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