HomeFAQsDoes the DNA need to be purified after the restriction digest, prior to CIP treatment?
FAQ: Does the DNA need to be purified after the restriction digest, prior to CIP treatment?
In most cases, CIP will work well in the reaction containing the restriction enzyme. Heat inactivate the restriction enzyme if possible. Some enzymes may bind tightly to the DNA ends and compete with CIP. Check the restriction enzyme data card for information regarding excessive DNA binding.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.