FAQ: What is the optimal heat shock time for this strain (NEB #C2984H and NEB #C2984I)?

Heat shock at 42ºC for 30-40 seconds results in the highest transformation efficiency for NEB Turbo competent E.coli (NEB #C2984H and NEB #C2984I). The transformation efficiency is not very sensitive to the changing the heat shock time within the range of 10-60 seconds. Expect approximately 20% loss in transformation efficiency when heat shocking for 80 seconds (see Figure on the main product page).
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    Tips for Successful Transformations with NEB Competent Cells

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