HomeFAQsHow should I calculate the Electrotransformation efficiency (C2986)?
FAQ: How should I calculate the Electrotransformation efficiency (C2986)?
Transformation efficiency is defined as the number of colony forming units (cfu) which would be produced by electrotransforming 1 µg of plasmid into a given volume of electrocompetent cells. For example, if a 10 pg transformation with pUC19 yields 100 colonies when 100 µl of a 1:100 dilution is plated, then the efficiency is 1.0 x 1010 cfu/µg pUC19 (100 cfu/10pg x 106 pg/µg x 1 ml/0.1 ml plated x 100 dilution).
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.