NEB will be closed Thursday, November 23, 2017 and Friday, November 24, 2017. Orders placed will process Monday, November 27, 2017 for delivery on Tuesday, November 28,2017.

FAQ: How should I calculate the Electrotransformation efficiency (C2986)?

Transformation efficiency is defined as the number of colony forming units (cfu) which would be produced by electrotransforming 1 µg of plasmid into a given volume of electrocompetent cells. For example, if a 10 pg transformation with pUC19 yields 100 colonies when 100 µl of a 1:100 dilution is plated, then the efficiency is 1.0 x 1010 cfu/µg pUC19 (100 cfu/10pg x 106 pg/µg x 1 ml/0.1 ml plated x 100 dilution).
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