HomeFAQsHow much enzyme should I use in the fragmentation reaction?
FAQ: How much enzyme should I use in the fragmentation reaction?
1 unit of RNase III fragments 100 ng of purified eukaryotic mRNA into 200 nucleotide length RNA fragments when incubated for 5 minutes at 37 °C. The amount of enzyme required for fragmentation depends on the amount of RNA input as well as the desired RNA fragment sizes.
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