FAQ: How long should I incubate cells on ice after DNA has been added (NEB #C2984H and NEB #C2984I)?

Incubating DNA with NEB Turbo competent cells on ice for 30 minutes is recommended. When tested with pUC19, an incubation time of just 2 minutes did not hurt the transformation efficiency (see Figure on the main product page).
  1. TransformationTips_VideoThumb_282x210_0616

    Tips for Successful Transformations with NEB Competent Cells

    Follow these tips to get superior results.