HomeFAQsHow can I generate a restriction enzyme site map for my sequence?
FAQ: How can I generate a restriction enzyme site map for my sequence?
NEBcutter®, a computer program for restriction enzyme site mapping is available on the NEB web site in the sidebar under "Favorite Tools". The program accepts sequences retrieved from a local file or GenBank. It also accepts an input sequence pasted into a designated field. When presented with a sequence, NEBcutter will find the largest open reading frames within the sequence and indicate those restriction enzymes that could be used to excise the gene. It also locates the positions of all restriction enzymes that cut only once within the sequence chosen. NEBcutter is also fully aware of the information on the methylation sensitivity of restriction enzymes and alerts a user to overlapping methylation by dam, dcm, etc.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.