FAQ: Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?

Exonuclease I can be used with Lambda Exonuclease (NEB# M0262) to clean up plasmid preps. Exonuclease III (NEB# M0206) and T7 Exonuclease (NEB# M0263) will also work, but will damage nicked plasmids.

Although Exonuclease I can be used, we recommend using Exonuclease V (RecBCD) to remove chromosomal DNA after plasmid prep.

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    What are exonucleases and their applications?

    Learn about exonucleases and some of their common applications.