Quick Tips - How can I optimize my ligation reaction?

This video gives tips on how to optimize your adapter ligation reaction.

Script

Customers often ask "What is the best way to optimize my ligation reaction?"

The best way to optimization your ligation reaction is to determine the ideal dilution factor for your adaptor. We do not recommend pre-mixing your adaptor with the ligation master mix, because this might yield adaptor dimers.

Remember, if you have any other questions, you can contact us at info@neb.com.

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