Have you wondered how you can optimize the fragmentation for RNA workflows in the NEBNext product line?
You can optimize the fragmentation by visualizing it on a bioanalyzer, but you have to clean up the sample first. The fragmentation happens in the first strand buffer, which contains magnesium at elevated temperatures. In order to run it on a bioanalyzer, clean it up with a 2.2x SPRI bead ratio. The 2.2x ratio ensures that you don’t lose the small fragments. If you then want to use your sample in library prep you have to bring it back into the first strand buffer.
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