Abundant transcripts in your sample? Where they come from and how to focus on sequences that matter to you. Part II
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The large dynamic range of transcript expression presents a challenge in whole-transcriptome sequencing. Highly expressed transcripts, their sources, and how they can hinder detection of informative transcripts will be discussed. We have optimized a method to enrich for transcripts of interest by eliminating specific RNAs before sequencing. This method is based on the hybridization of probes to unwanted RNAs followed by enzymatic degradation of the targeted RNAs and probes. In this webinar we present our latest solutions, including a customization option, to enrich for RNAs of interest across diverse species.
In part 2, Bradley W. Langhorst, Deyra N. Rodriguez and Keerthana Krishnan will discuss a solution for removal of rRNA from a wide variety of bacterial species. Furthermore, they will introduce a new customizable approach to remove any RNA of interest from your favorite sample, and demonstrate a user-friendly, easily accessible web tool to enable custom probe design. Probes can be designed to target RNAs in single species, multiple species, or used to supplement existing probe sets. After the tool demonstration, they will provide guidelines for evaluating your custom design.
Please join us after the webinar for an opportunity to engage in discussion and ask questions of the NEB experts.