Bacterial Cells: E. coli or Salmonella typhimurium
Cell Data
per cell
per liter at 109 cells per ml
Wet Weight
9.5 X 10-13 g
0.95 g
Dry Weight
2.8 X 10-13 g
0.28 g
Total Protein
1.55 X 10-13 g
0.15 g
Volume
1.15 µm3 = 1 femtoliter
Protein Conc. in the cell: 135 mg/ml
Theoretical maximum yield for a 1 liter culture (109 cells /ml) if protein of interest is:
0.1% of total protein: 150 µg/liter
2.0% of total protein: 3 mg/liter
50.0% of total protein: 75 mg/liter
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