Competent E. coli Genome Sequences Tool
Complete genome sequences of most E. coli competent cell strains sold by NEB are now available in GenBank at NCBI using the accession numbers below. Some strains have associated episomes, which have separate accession numbers. By default, NCBI (National Center for Biotechnology Information) may not display the entire record. In this case, go to the “Customize view” bar on the NCBI page to open the drop-down menu, then click “Customize” and select the desired options. A separate toggle on the upper left of the NCBI page will switch to FASTA format. Consult the NCBI website for additional options.
Strain Name | Catalog No. | Genome Accession | Episome Accession | Episome |
---|---|---|---|---|
Cloning Strains | ||||
NEB® 5-alpha | C2987 | CP017100 | n/a | |
NEB 5-alpha F'Iq | C2992 | CP053607 | CP053608 | F’ (lacIq) |
NEB 10-beta | C3019 | CP053604 | n/a | |
NEB Turbo | C2984 | CP053605 | CP053606 | F’ (lacIq) |
dam-/dcm- | C2925 | CP053603 | n/a | |
Protein Expression Strains | ||||
SHuffle® T7 | C3026 | CP014272 | CP014273 | F’ (lacIq) |
SHuffle T7 Express | C3029 | CP014269 | n/a | |
BL21(DE3) | C2527 | CP053602 | n/a | |
BL21 | C2530 | CP053601 | n/a | |
NiCo21(DE3) | C2529 | CP053600 | n/a | |
NEBExpress® | C2523 | CP053599 | n/a | |
NEBExpress Iq | C3037 | CP053592 | CP053593 | MiniF_lacIq |
T7 Express | C2566 | CP014268 | n/a | |
T7 Express lysY | C3010 | CP053597 | CP053598 | MiniF_lysY |
T7 Express lysY Iq | C3013 | CP053595 | CP053596 | MiniF_lysY/lacIq |
T7 Express Crystal (discontinued) | C3022 | CP053594 | n/a |