Protocol for RecJf (NEB #M0264)

RecJf is a single-stranded DNA specific exonuclease that catalyzes the removal of deoxynucleotide monophosphates from DNA in the 5´ → 3´ direction.

  1. Set-up a typical reaction as follows:
DNA Up to 1 µg
NEBuffer 2 (10X) 5 µl (1X)
RecJf 1 µl (30 units)
Nuclease-free H2O (NEB #B1500S) up to 50 µl
  1. Incubate at 37°C for 20 minutes.

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