Protocol for Regeneration of Oligo d(T)25 beads
Oligo d(T)25 beads can be regenerated and used to isolate RNA from a different source.
- Add 0.1 N NaOH to the beads and incubate at room temperature with agitation for 5 minutes.
- Wash the beads three times with elution buffer (or sterile RNAse-free H2O),
- Equilibrate the beads by washing three times with sterile RNase-free 1X PBS (pH 7.4) containing 0.05% Tween 20
- Store in sterile RNase-free 1X PBS (pH 7.4) containing 0.05% Tween 20 and 0.02% NaN3.