Perform Second Strand cDNA Synthesis (E7530)

Protocol

1. Add the following reagents to the First Strand Synthesis reaction (20 μl):
   
   Nuclease-free water   48 μl
   Second Strand Synthesis Reaction Buffer (10X)   8 μl
   Second Strand Synthesis Enzyme Mix   4 μl
   ----------------------------------------------------------------------
   Total volume   80 μl
   
   
2. Mix thoroughly by gentle pipetting.
   
   
3. Incubate in a thermal cycler for 1 hour at 16°C, with heated lid set at ≤ 40°C.