Use with CLIP-Cell PF
CLIP-Tag fusion proteins can be expressed by transient or by stable transfection. For expression of fusion proteins with CLIP-Tag refer to instructions supplied with the CLIP-Tag plasmids and the guidelines from your expression plasmid provider. For cell culture and transfection methods, refer to established protocols.
Dissolve one vial of CLIP-Tag substrate (50 nmol) in 50 µl of DMSO to yield a labeling stock solution of 1 mM CLIP-Tag substrate in DMSO. Mix for 10 minutes until all the CLIP-Tag substrate is dissolved. Store this stock solution in the dark at +4°C, or for extended storage at -20°C. Different stock concentrations can be made, depending on your requirements. The substrate is soluble in DMSO up to at least 10 mM.