NEBNext dA-Tailing Module Protocol (E6040)


Starting Material: 1–5 μg of end repaired, blunt DNA (100–1000 bp)in 37 μl H2O.


  1. Mix the following components in a sterile microfuge tube:

    End Repaired, Blunt DNA:   42 μl
    NEBNext dA-Tailing Reaction Buffer (10X):   5 μl
    Klenow Fragment (3´→ 5´ exo):   3 μl
    Total volume:   50 μl
  2. Incubate in a thermal cycler for 30 minutes at 37°C.
  3. Purify DNA sample on one column and elute in 25 μl of sterile dH2O or elution buffer.