Protocol I (Luminometers without injectors) (E3308)

Protocol

1. Prepare the GLuc assay solution (e.g. 100 samples) by adding 50 μl of BioLux GLuc Flex Substrate and 0.8 ml of BioLux GLuc Flex Stabilizer to 5 ml of BioLux GLuc Flex Assay Buffer.
2. Mix well by inverting the tube several times (Do not vortex).
3. Incubate at room temperature for 25 minutes (protect from light in a tightly capped tube/bottle) before adding to the sample.
4. Set the luminometer for 2–10 seconds of integration.
5. Pipet samples (5–20 μl per well) into a 96-well white (opaque) or black plate, or a luminometer tube.
6. Add the assay Solution (50 μl per well) to all samples.
7. Incubate at room temperature for 35–40 seconds (refer to Usage Notes) and proceed with the measurement.