Protocol for a Typical miRNA Methylation Reaction (M0228)
| 10X NEBuffer | 2.5 µl |
| 3.2 mM SAM | 1 µl |
| miRNA duplex (100–500 ng | X |
| H2O | X |
| RNase inhibitor (optional) | 20 units |
| HEN 1 RNA Methyltransferase (50 units/µl) | 1 μl |
| Total reaction volume | 25 μl |
| 10X NEBuffer | 2.5 µl |
| 3.2 mM SAM | 1 µl |
| miRNA duplex (100–500 ng | X |
| H2O | X |
| RNase inhibitor (optional) | 20 units |
| HEN 1 RNA Methyltransferase (50 units/µl) | 1 μl |
| Total reaction volume | 25 μl |
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.