NEBNext DNA Sample Prep Master Mix Set for 454 Quick Ligation Module Protocol (E6070)

Protocol

1. Mix the following components in a sterile microfuge tube: 
   End Repaired, Blunt or dA-Tailed DNA:  30 μl
   Quick Ligation Reaction Buffer (5X):  10 μl
   DNA Adaptors(not provided please use adaptors appropriate to specific application):  5 μl
   Quick T4 DNA Ligase:  5 μl 
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   Total volume:  50 μl
2. Incubate in a thermal cycler for 15 minutes at 20°C.
3. Purify DNA sample on one column and elute in 25 μl of sterile dH₂O or elution buffer.