NEBNext DNA Sample Prep Master Mix Set for 454 End Repair Module Protocol (E6070)

Introduction

Starting Material: 1–5 μg of DNA Fragmented to 100–1000 bp in ≤ 85 μl

Protocol

1. Mix the following components in a sterile microfuge tube: 
   Fragmented DNA:  1–85 μl
   NEBNext End Repair Reaction Buffer (10X):  10 μl
   NEBNext End Repair Enzyme Mix:  5 μl
   Sterile H₂O for a final volume of 100 μl:  variable 
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   Total volume:  100 μl
2. Incubate in a thermal cycler for 30 minutes at 20°C.
3. Purify DNA sample on one column and elute in 30 μl of sterile dH₂O or elution buffer.