NEBNext End Repair Module Protocol (E6050)


Starting Material: 1-5 μg of DNA Fragmented to 100-1000 bp in ≤ 85 μl


  1. Mix the following components in a sterile microfuge tube:

    Fragmented DNA variable
    NEBNext End Repair Reaction Buffer (10X) 10 μl
    NEBNext End Repair Enzyme Mix 5 μl
    Sterile H2O for a final volume of 100 μl variable
    Total volume 100 μl

  2. Incubate in a thermocycler for 30 minutes at 20°C with heated lid set to 30°C (or off).

  3. Purify DNA Sample using AMPure XP or SPRIselect beads.
Note: for details how this module is used in the NEBNext Library Prep for Illumina workflow, please see manual for NEBNext DNA library Prep Master Mix Set for Illumina (NEB #E6040).