NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090)

Protocol

1. Starting Material: 0.5 μg of DNA Fragmented to 100–1000 bp in 16 μl of TE.
   
   In a 1.7 ml micro-centrifuge tube add:

   End Prep Enzyme Mix	1.0 μl
   End Repair Reaction Buffer (10X)	2.5 μl
   Nuclease -free Water	5.5 μl
   Total	9.0 μl

   
   
   
2. Mix by pipetting and add to the 16 μl fragmented DNA sample.
   
   
3. Vortex briefly to mix, followed by a quick spin to collect all liquid from the sides of the tube.
   
   
4. In a thermocycler, with the heated lid on, run the following program: 
   20 minutes @ 25°C 
   20 minutes @ 72°C 
   Hold at 4°C